Part:BBa_K3098008:Design

GST+PgD5

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 688
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 784
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 85
  

Design Notes

The accuracy of the enzyme that cut GST need to be test.

Source

GST tag is from the vector pGEX6P and has been used for research commonly. PgD5 is from Picea glauca genome